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二、主要学术成就<=
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2.1 <=
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申请人致力于从生物力学（mechanobiology）角度研究细胞极性和感知周围物理环境的机制。申请人以共同第一作者(排一)和共同作者身份在Nature Cell Biology（NCB）杂志发表了两篇论文。申请人发表的NCB第一作者论文共被引用162次（Google Scholar统计）。该研究揭示了单个细胞里的细胞骨架能自发形成左右不对称（chiral）的网络结构，犹如是细胞里的指南针, 帮助细胞区分左右方向。该研究首次阐明了细胞骨架左右不对称的特殊结构是源于formin蛋白和肌动蛋白之间的相互协同在其中的调控机制。其中提到的分子机制也有助于了解组织形态形成，在组织工程学、类器官模型和药物测试中具有潜在的应用价值。申请人在2019成功地获得新加坡国家医学研究委颁发的年轻个人研究基金, 独立主持30万新加坡元的基金。
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2.2主要学术成 =
489;、贡献、创新点科学=
;价值或社会经济意义&#=
21450;影响力（不&#=
36229;过3000字）
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申请人目前在新加坡国立大学生物力学卓越研究所担任高级研究员，合作导师为细胞生物与力学领域著名科学家Alexander D. Bershadsky（以色列魏斯曼研究所兼聘教授）教授。力学生物学对细胞的机械性能和功能都有着重要作用，申请人一直以来致力于从生物力学 (mechanobiology)角度研究细胞如何感知周围物理环境 (mechanosensing) 以及肌动蛋白细胞骨架 (actin cytoskeleton) 为应对细胞所处的物理环境如何自发形成 (self-organized) 网络结构。申请人也同时致力于开发基于图像分析的表型组学技术（image-based phenomics）来进行转化医学研究。 突出成果小结：申请人在生物力学和细胞骨架研究领域取得多项代表性研究成果，以第一作者和共同作者身份在Nature Cell Biology (NCB) 杂志发表两篇研究论文。申请人在细胞骨架对称破缺 (symmetry breaking) 领域发表的第一作者论文共被引用162次（Google Scholar统计）。该研究通过细胞表型的观察，揭示了单个细胞里的细胞骨架能自发形成左右不对称 (left-right asymmetric/chiral) 的网络结构，犹如是细胞里的指南针，帮助细胞区分左右方向。该研究首次阐明了细胞骨架左右不对称的特殊结构是源于formin蛋白和肌动蛋白之间的相互协同作用。该研究成果不仅有助于理解组织形态发生 (tissue morphogenesis)的基础理论，在组织工程学、类器官模型和药物测试方面也具有潜在的应用价值。 申请人的主要学术创新成果综述如下： 1) 揭示了单细胞具有分辨左右 (left-right determination) 的能力，并首次解析出该分辨能力依赖于formin蛋白和肌动蛋白分子之间的协同作用。 左右不对称性 (left-right asymmetry aka chirality) 是大自然里中奇妙的生物现象，包括体内器官的位置、大脑左右半球、优选右转的粘菌、以及蜗牛壳的卷绕。保证器官不对称的形成对健康至关重要，左右不对称性发生缺陷会导致胚胎死亡或先天性心脏病等疾病。因此，深入理解生命过程中左右极性的产生是一个重要的基础科学问题。目前人们已经认识到，胚胎发育过程中打破左右对称的机制是机械性质的 (dependent on mechanical forces)。但一个尚未解决的关键科学问题是：存在左右分别的细胞中，最初左右轴 (left-right axis)是如何启始并进而导致不对称机械力的产生？为了回答这一关键问题，申请人展开多学科交叉研究，利用 micropatterning技术把单个成纤维细胞 (fibroblast) 限制为圆形，以产生一个各向同性 (isotropic) 的环境。申请人发现，细胞骨架先自发形成径向对称 (radially symmetrical) 的网络结构，然后演变成有左右极性形态的网络结构。申请人进一步鉴定出formin蛋白是细胞骨架呈现左右极性的主要调控因子，并阐明formin蛋白通过与具有右旋性质的肌动蛋白丝进行协同，产生有偏向的旋转扭矩，来导向细胞左右轴的方向。相关工作申请人以第一作者（共一排一）及封面论文的形式发表于Nature Cell Biology (Tee et al., 2015)。 该文章被Faculty of 1000推荐；发表当年即被著名杂志Science在“This week in other journals” 栏目进行正面评论(Science 348 (6231), page 197-198, 2015)；也被Current Biology杂志在Dispatch 栏目正面评述(Mogilner and Fogelson, 2015)；迄今为止已被引用162次（根据Google Scholar）。这项研究的影响不限于左右不对称的领域，也被生命科学、生物物理和组织工程等领域最新发表的文章所引用。根据Altmetric Attention Score (数据科学评分系统旨在反映研究成果的覆盖范围和受欢迎程度, 该数据科学评分系统也被顶级国际刊物Science和Nature采用)，该文章曾在相同时段中所有可追寻的发布期刊文章中，排前98% (high attention score)。 申请人的发现引起了学术界的广泛关注，并荣获2015亚太生物力学协会颁发的山口生物力学奖章 (Yamaguchi Medal in Biomechanics)，并且被许多国际会议（Asian Pacific Conference on Biomechanics；Asia Pacific International Congress of Anatomists and The American Society For Cell Biology (ASCB) Annual meeting）邀请做口头报告。这一发现也被诸多国际知名科学新闻媒体进行了专题报道，其中包括：英国的MRC 医学科学研究所（Institute of Medical Sciences）（以题为“Beautiful Asymmetry”的图片对这一发现进行了高亮推荐）、法国的La Recherche杂志、以及美国的Eurek Alert! 和Science News杂志。以这一具有开创性的工作为基础，申请人在2019成功地获得新加坡国家医学研究委员会（National Medical Research Council）颁发的青年研究基金 (Young Individual Research Grant), 独立主持30万新加坡元（约合150万人民币）的基金。在此研究基础上，申请人作为共同作者，在Nature Cell Biology (Hu et al., 2017) 上发表了关于肌球蛋白-II (myosin-II) 细丝合成的自组织性的论文。 2) 利用“基于图像的表型组学”技术 (image-based phenomics)，发现formin mDia1蛋白是单细胞中调控左右极性的关键分子，并且揭示了上皮-间质转化过程中细胞骨架的多维相变 (multidimensional phase transition)。 上皮-间充质转换 (epithelial to mesenchymal transition，EMT) 在胚胎发育、伤口愈合和肿瘤转移中起着重要作用。申请人的NCB（2015）论文证明了formin蛋白能影响单细胞中的左右极性轴定向。在此基础上，申请人进一步鉴定了formin蛋白家族里的 mDia1是决定着上皮细胞骨架呈现左右极性的关键分子，并深入解析了EMT过程中细胞骨架的变化。这一发现对筛选调控EMT过程的新型药物及基因治疗手段提供理论依、据具有重要意义。基于以上成果，申请人以共同通讯作者在Journal of Cell Science期刊发表, 也被选为当期的封面论文。 根据Altmetric Attention Score，该文章曾在相同时段中所有可追寻的发布期刊文章中，排前88% (high attention score)。申请人由于该发现受邀在由美国生物物理学会赞助的生物物理交流日（吉隆坡，2019年9月17号）作报告。 3) 鉴定了kinectin蛋白的新细胞学功能以及微管调控调控粘着斑的新机理。 Kinectin是一种内质网膜整合蛋白，可以通过与驱动蛋白互作，在微管上对内质网进行延展。在包括贝塞特氏症、肝细胞癌、口腔鳞状细胞癌和帕金森病在内的多种疾病中，kinectin被认为是潜在生物标记物。但目前对这一重要分子的细胞学功能却知之甚少。通过观察和比较内质网的动态变化，申请人发现kinectin蛋白通过调控内质网来影响在粘着斑的生长。该研究不仅揭示了内质网介入粘着斑成熟的新机制，也在生物学细胞骨架和动力领域提出了新的理论观点并引发了学术界的关注。这篇文章被选为Journal of Cell Science编辑的亮点之一，也获得Faculty of 1000 推荐。Faculty of 1000评价：在此研究之前，微管只被发现与粘着斑的解体有关，而本研究发现微管在粘着斑成熟过程中也发挥重要作用。申请人也受邀在美国举办的国际戈登研究研讨会(Gordon Research Seminar, USA) 中报告该工作，并获得新加坡国立大学Yong Loo Lin医学院的 “最佳学生论文奖”和 “最佳口头演讲奖”。
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三、岗位工作思路&=
#21450;预期目标
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研究方向和思路 Cell polarity refers to the asymmetric organization of cellular components such as the cell membrane, cytoskeleton or organelles. It is a fundamental property of all cells in our body, thereby allowing each cell type to perform their unique specialized functions such as nutrient transport, signaling, motility and mechanosensing. Establishing and maintaining cell polarity is critical for development and homeostasis, with the loss of cell polarity recognized as a hallmark of pathology and cancer. In multicellular organisms, cells integrate information from numerous stimuli such as signaling, physical stress, cell-cell contacts and extracellular matrix microenvironment to define their polarity axis. Understanding the individual contribution of each factor in defining cell polarity has wide-ranging benefits, such as identification of critical cell polarity proteins as proto-oncogenes or tumor suppressors, use of cell polarity markers for drug screenings and intelligent tissue-engineering design to recreate highly functional tissue in vitro. The complexity of the intracellular environment is one of the main challenges in unraveling the individual role each stimulus plays in organizing cell polarity. I plan to overcome this challenge by using microfabrication tools which would allow me to precisely control the cell morphology and the external cues to be presented to the cells. Using micropatterned substrate to confine cells onto circular fibronectin islands, I revealed that a mechanism involving asymmetric interaction between the formin molecule and helical actin filament is central in defining the left-right polarity axis in single cell (Nature Cell Biology, 2015). I propose that through examining cellular behaviors in well-defined microenvironments, we will then be able to understand how cells achieve homeostasis, and identify biologically relevant alterations during disease development. Following the above principles and methodology, we successfully identified key actin cytoskeletal changes in cells before and after epithelial-to-mesenchymal transition (EMT)(Journal of Cell Science, 2019), which is a process associated with development, wound healing and tumor metastasis. Building on my initial discovery of a left-right asymmetric organization of the actin cytoskeleton in single cell and an established experimental set-up to analyze cell behaviors, I plan 3 specific aims in this proposal for future research aim at understanding how tissue (目标1) and neuronal cells (目标2) organize their left-right polarity axes, and to exploit the phenomenon of left-right asymmetry observed in cardiomyocytes for screening putative cardiotoxic compound (目标3). 目标1. To investigate the relationship between mechanism(s) controlling single cell chirality and left-right asymmetric morphogenesis of tissue. At first glance, the superficial symmetry of the human body beguiles the fact that the body’s natural left-right asymmetry beneath the skin surface is crucially important to our health. Error in proper left-right patterning is known as heterotaxy, a complex heritable disorder present in approximately 1 in 10,000 births and is characterized by randomization of visceral organs positioning. This may be lethal and mostly contribute to congenital heart condition. Many controversies and gaps in knowledge remain in the field, one of the key questions is “what is the relationship between establishment of left-right cell polarity (seen in behavior of single cell) and tissue or body-plan asymmetry?”. 1.1 To establish a link between left-right cell polarity found in single cell and tissue-scale left-right morphogenesis. Collective cell migration plays an important role in shaping tissues during organogenesis. In living tissue, cell movements are constrained by neighboring cells and surrounding extracellular matrix. To mimic the spatial constraints that cells encounter within tissues in an experimentally controlled way, we plan to culture fibroblast cells on fibronectin-coated island surrounded by non-adhesive regions, so that cells’ attachment and migration were confined to the adhesive areas. Preliminary study, using large rectangle micropattern of an area for ~100 cells, revealed that fibroblasts movements in this spatially constrained environment transit from a random-walk fashion to a cohort collective migration that demonstrated chirality. We have already identified several drug treatments and gene knockdowns that would reverse chirality in single fibroblast cell (NCB 2015 and unpublished preliminary data). We plan to test if the same manipulations affecting single cell chirality would also reverse the direction of chiral collective cell migration. 1.2 Investigating the mechanics and factors regulating collective cell migration and emergence of tissue-scale chirality. Collective cell migration on large rectangle micropattern appears to “flow” as a continuous sheet. Such flow has also been reported in vivo during tissue development and in cancer. Here, we will investigate the orientation and the dynamics of fibroblasts within these rectangle micropatterns and analyze their behavior to understand the mechanics of collective movement and the process of symmetry-breaking. We plan to monitor the velocity fields and orientation angles, in time and space, of individual cells within the rectangle micropattern to reveal the group decision-making process that leads to chiral symmetry-breaking. We will also study how these quantitative features change following treatments that reverse the direction of tissue-scale chirality. 目标2. To develop HiT-P (High-Throughput Phenomics) platform: image-based phenomics for basic science and translational research. 2.1 To develop an automated high-throughput imaging and image analysis platform for quantitative characterization of cellular phenotypes (phenomics). Cells on micropatterned substrate is advantageous for high-throughput image acquisition: (1) cells will not move out of the imaging field of view, and (2) acquisition can be automated using motorized microscope stage that moves precisely to each micropattern. Although altered phenotypes can reliably be associated with altered gene functions, systematic analysis of phenotypes relationship to gene expression remains nascent. The proposed development of the HiT-P platform in our study intends to fill this gap. It is now timely to exploit the advantages of micropattern-enhanced research: (1) predictable phenotypes that can be described and quantified, (2) engineering designs to control cell behavior, and (3) lowering sampling requirement to assess the population. The HiT-P platform will be used to address research questions described in this proposal. 2.2 To investigate the role of left-right cell polarity proteins in determining neuronal polarity. The relevance of left-right asymmetry to physiology and health is undeniable. Besides the chiral behavior in fibroblast as discussed in 目标1 and the left-right asymmetric heart organ as discussed later in 目标3, the brain also has distinct left and right hemispheres, each is dominant in processing specific cognitive tasks. Anomalies of left-right asymmetries in the brain has been associated with neurological diseases. Neurons are highly polarized cells harboring elaborate dendritic extensions, which are actin-based filopodia-like structures. Dendritic extensions have been reported to exhibit chiral turning in culture. Here, we will screen the effect on neuronal dendrites turning following knockdown of identified genes that affect actin cytoskeletal chirality in fibroblasts (from 目标1 ). 目标3. To exploit the phenomenon of left-right asymmetry observed in cardiomyocytes for screening putative cardiotoxic compound. Drug-induced cardiotoxicity, which can lead to heart failure and even death, is a leading cause of drug withdrawal. Hence, it is imperative to detect as early as possible whether a newly developed drug compound has cardiotoxic side effects. Here, we want to explore if cardiomyocytes perform better in their functionality assays when cultured on micropatterned substrate that promote their collective left-right asymmetric behavior, and potentially to use chirality as an endpoint in drug screening. 3.1 Emergence of intrinsic cellular chirality in cardiomyocytes. The heart is an asymmetric organ morph from left-right asymmetric collective cell movements during development. Explanted heart retained chiral looping behavior ex vivo, except in conditions with actin-disrupting drugs, suggesting that chirality is an intrinsic tissue property which depends on the actin cytoskeleton. In view that intrinsic cellular mechanism controls heart left-right morphogenesis, in vitro environment using micropatterns as planned in this proposal would be a suitable experimental setup for studying chiral morphogenesis of cardiomyocytes from the molecular to tissue level. This is supported by preliminary data that rat cardiomyocytes (H9C2), like human fibroblast, showed actin cytoskeleton chirality. To evalsuate the relationship between cardiomyocyte cell chirality and dysregulation of cardiomyocyte health, we plan to investigate how cardiomyocytes chirality can be altered in the following conditions – (1) differentiated versus undifferentiated cell state, (2) known genetic alterations that lead to heart defects, and (3) exposure to known cardiotoxic drugs. 3.2 Cell functionality and drug response in cardiomyocytes culture exhibiting left-right asymmetric behavior. In tissue engineering, biomaterials are often designed to target biocompatibility, but neglect to recapitulate the physiological L-R asymmetric property of a tissue. Here, we hypothesize that cardiomyocytes perform better in cardiac functionality assays when cultured in conditions that promote the emergence of their chiral behavior. To test this hypothesis, we will compare the cell functionalities of cardiomyocytes cell group cultured on large rectangle micropatterns versus on isotropic circle micropatterns. We will also test if loss of left-right asymmetric collective cell migration could be used as endpoint in predicting drug-induced cardiotoxicity by examining collective cell migratory behavior following exposure to known cardiotoxic drugs. 预期贡献 1） 证明肌动蛋白的分子手性(molecular chirality)是主导着细胞和组织出现左右极性(left-right asymmetric)结构的基础。 2） 结合微图形构建(micropatterning)和显微成像技术，来系统、量化地研究基因信息是如何被转化成细胞形态指令，并且分析这些指令在病态的情况下会发生怎样的变化。 3） 建立一个使用细胞和组织左右极性现象来作为预测药物的心脏毒性的科技应用，以用来优化和开发新的心脏药物。 4） 争取在3-5年的时间里陆续实现研究计划里的目标，并争取在顶级国际刊物上发表论文。 5） 与浙江大学教师和国际专家一起开始新的研究生课程，介绍生物力学基础知识和研究。 6） 与新加坡国立大学研究伙伴保持合作，通过会议和学生交流来增强研究能力。
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四&=
#12289;任现职=
0197;来近５年(根&#=
25454;所在院系任职基本Ĉ=
65;件要求的年限填写)主要业绩
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4.1教学与人才=
521;养情况
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1、共开设课 =
243; １ 门，授课ਲ=
2;数共计 16 学时。其中=
;本科生课程 0 门，=
课程教学时数 0 学时，具体ঀ=
0;课情况如下：
教学=
年度，课程名称，授=
5838;对象，学生数，本ߟ=
4;学时数/课程总学时数，考核=
结果
1)
2018-2019, MB5102: Topics in Mechanobiology, 研究生, 12, 8/36,
2)
2017-2018, MB5102: Topics in Mechanobiology, 研究生, 12, 8/36,
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2、指导本科 =
983;毕业论文（设计） 3 人（请列=
出姓名、专业、年级=
5289;
1) Yeap Sin Hui, 生命科学, 一年级
2) Oen Siow Ween, 生命科学, 一年级
3) Yong Xianbin, 生命科学, 一年级
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3、指导研究 =
983; 2 名 (请列出研究生姓名、=
研究生类型、专业、=
4180;级)
1) Salma Jalal, 博士研究生, 力学生物学, 一年级
2) Galia Sakaeva, 博士研究生, 细胞生物学和生物物理学, 一年级
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4.2代表性论文=
289;著作情况
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1、共发表论=
991; 6 篇，其中作=
为第一作者或通讯作=
2773; 3 篇。
请按照您认为最具=
195;表性、重要性或影响=
;力的顺序列出：
=
所有作者姓名（通讯=
0316;者名字前用“*”标示），论ă=
91;题目，发表期刊名称=
，出版年月，卷，期=
5292;起止页码，检索收ঈ=
5;情况，期刊影响因子&=
#65292;他引次数，期刊级=
035;
1) Tee Y. H.^, Shemesh T.^, Thiagarajan V., Hariadi R. F., Anderson K. L., Page C., Volkmann N., Hanein D., Sivaramakrishnan S., Kozlov M. M., and Bershadsky A.D*, Cellular chirality arising from the self-organization of the actin cytoskeleton, Nature Cell Biology, 2015年4月, 17, 4, 445-457, SCI, 20.001, 111, 其他
2) Hu S., Dasbiswas K., Guo Z., Tee Y.H., Thiagarajan V., Hersen P., Chew T., Safran S. A., Zaidal-Bar R. *, and Bershadsky A.D*, Long range self-organization of cytoskeletal myosin II filament stacks, Nature Cell Biology, 2017年2月, 19, 2, 133-141, SCI, 20.46, 44, 其他
3) Jalal S., Shi S., Acharya V., Huang R.Y., Viasnoff V., Bershadsky A.D.*, and Tee Y.H*, Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement, Journal of Cell Science, 2019年3月, 132, 5, 1-14, SCI, 4.831, 0, 其他
4) Hu S., Tee Y.H., Kabla A., Zaidel-Bar R., Bershadsky A.D., and Hersen P*, Structured illumination microscopy reveals that focal adhesions are composed of linear subunits, Cytoskeleton, 2015年5月, 72, 5, 235-245, SCI, 2.893, 11, 其他
5) Lou Y. R.*, Toh T. C., Tee Y.H., and Yu. H, 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells, Scientific Reports, 2017年2月, 7, 未知, 1-12, SCI, 4.609, 4, 其他
6) Tee Y.H. and Bershadsky A.D*, Actin retrograde flow in permeabilized cells: myosin-II driven centripetal movement of transverse arcs, Bio-protocol, 2016年3月, 6, 5, -, , , , 其他
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945;材共 0 本，总字数=
;为 0 万字，其ߑ=
3;为主编、副主编出版&=
#20840;国统编教材 0 本，省部重=
;点、规划教材共 0 本：
=
所有作者姓名，书名=
5292;著作类型，出版地ᦁ=
2;出版社名称，出版年&=
#26376;，个人字数/总字数，主编/副主编
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4.3主要科研、=
945;改项目情况
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1、&#=
20849;参加项目 1 项，=
其中纵向项目 1 项，=
横向项目 0 项<=
/b>；
主持=
项目到校总经费 0 万元，其中=
纵向项目到校经费 0 万元，横×=
21;项目到校经费 0 万元。
2、作为项目负责人=
5;担项目 1 项，=
其中纵向项目 1 项，=
横向项目 0 项。=
请按=
您认为最具代表性、=
7325;要性或影响力的顺ॴ=
7;列出：
=
项目名称，项目类别=
5292;项目性质，项目来=
4;，项目编号，本人主&=
#25345;到校经费/项目总经费(万元)，起始年月， =
456;止年月，项目成员
1) Actin cytoskeleton driven intrinsic chirality – a role in collective cell migration and in tissue engineering application, 其他(), 纵向, NMRC OF-YIRG (新加坡国家医学研究委员会, 开放式基金-青年个人研究基金), OFYIRG18May-0041, 300,000新元/300,000新元, 2019年3月, 2022年2月, Tee Yee Han, Yan Jie, Felix Marin Margadanat
2) New optical platform to visualize mechanics of cellular self-organization, 其他(), 纵向, A*STAR – JST Joint Grant Call (新加坡科技研究局 - 日本科技机构), 1514324022, 所在实验室主持的为150,000新元/300,000新元, 2016年1月, 2018年12月, Fumio Motegi, Alexander Bershadsky, Tee Yee Han
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4.4获得重要成=
524;奖励情况
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共获=
成果奖 =
项&#=
65292;其中教材奖 <=
span
style=3D'font-family:SimSun'>项&#=
65292;教学成果奖 项&#=
65292;科研成果奖 <=
span
style=3D'font-family:SimSun'>项。
请按=
您认为最具代表性、=
7325;要性或影响力的顺ॴ=
7;列出=
：
=
所有获奖人员姓名，=
9033;目名称，奖励名称ᦁ=
2;获奖级别，授奖单位&=
#65292;获奖年月，本人排=
517;/总人数
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4.5担任国际期刊Ņ=
34;委、国际学术会议重=
要职务及在国际学术=
0250;议全会报告、特邀ঢ়=
3;告情况
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(1) 国际学术会议口头报告 2019 "Actin polymerization and filament cross-linking determine chirality of individual cells and cell groups" CENTURI 2019 Scientific Meeting - Self-organization in multicellular systems Institut d'Études Scientifiques de Cargèse, Corsica, France. 30 Sept - 4 October (Invited Speaker) "The yin-yang of cell and tissue morphogenesis" The second International Youth Scholars Forum School of Basic Medical Sciences, ZJU, Hangzhou, China. 18-19 January 2018 (Invited Speaker) "Emergence of left-right asymmetry in single cell and cell groups" BIT’s 8th Annual World Congress of Molecular and Cell Biology Fukuoka, Japan. 14-16 October 2016 (Invited Speaker) "Self-organization of the actin cytoskeleton" 7th Asia Pacific International Congress of Anatomists Singapore, Singapore. 17-20 March 2015 (Award Winner) "Cellular chirality arising from the self-organization of the actin cytoskeleton" The 8th Asian Pacific Conference on Biomechanics Sapporo, Japan. 16-19 September 2014 "Cellular chirality arising from the self-organization of the actin cytoskeleton" The American Society For Cell Biology (ASCB) Annual meeting Philadelphia, Pennsylvania, USA. 6-10 December (2) Public Engagement · Guest Speaker (2019). GLEAP Seoul National University Outreach Visit. Mechanobiology Institute. Singapore. 9 January. (3) Service as a reviewer Number: 1 research article Publisher: American Chemical Society (ACS)，1155 16th ST, NW, Washington, DC 20036 USA Journal: ACS Biomaterials Science & Engineering, Impact Factor: 4.511
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4.6&=
#33719;得专利情况
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共获专利 项，其中发=
;明专利 项。
请按您认为=
368;具代表性、重要性或=
;影响力的顺序列出：
=
所有专利人员姓名，=
9987;利名称，专利类型ᦁ=
2;专利授权国，专利号&=
#65292;授权公告年月，本=
154;排名/总人数
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4.7其他获奖及荣Ţ=
65;情况=
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1. Yamaguchi Medal in Biomechanics (Cellular and Molecular Biomechanics). The 8th Asian-Pacific Conference on Biomechanics Hokkaido University, Sapporo, Japan. 16-19 September 2015 2. Travel Fellowship. Workshop on Mechanics and Growth of Tissues: From Development to Cancer CURIE Institute, Paris, France. 13-16 January 2014 3. Best Student Publication Award. The 3rd Physiology Graduate Students' Symposium Department of Physiology, NUS, Singapore. January 2011 4. Best Oral Presentation Award. 1st Graduate Scientific Congress School of Medicine, NUS, Singapore. January 2011 5. 1st place (video category). 4th MBI Microphotography Competition Mechanobiology Institute, Singapore. August 2013 6. 2nd place. 1st MBI Microphotography Competition Mechanobiology Institute, Singapore. April 2011
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4.8 社会服务及兼ň=
44;等情况=
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1. 担任ACS Biomaterials Science & Engineering杂志审稿人。 2. 作为志愿者讲授公共课程：
（1）Volunteer Speaker (2019). GLEAP Seoul National University Outreach Visit. Mechanobiology Institute. Singapore. 9 January. （2）Volunteer Lecturer (2013). Life Sciences Seminar Series. School of Life Sciences and Chemical Technology. Ngee Ann Polytechnic. Singapore. 19 July
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五、未列入业ń=
89;统计的其他能反映学=
术研究水平的突出业=
2489;
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无。
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个人承诺
本人保证：所从事的学术研究符合学术道德规范要求；所提供的材料客观真实；符合申报要求和任职条件。
承诺人：<=
b>![3D"zf_image"](3D"file2216.files/image004.gif") &nbs=
p;
2019年10月08日
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上述材料均已审核&#=
65292;内容真实，与证明Ĉ=
48;料原件相符。
审核人：![3D"file_image"](3D"file2216.files/image006.gif")
2019年10月08日
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![3Dewm](3D"file2216.files/image002.gif")